Villarreal holding midfielder Rodrigo "Rodri" Hernandez also earned a first call-up, making the leap from the under-21s to the first team.
Lopetegui said of the newcomers: "We felt it was the right time for them to come in and take a look at them".
Spain open their World Cup campaign with an Iberian derby against neighbours Portugal on June 15 before facing Iran and Morocco.
The Spaniard spent just one season back in Madrid, scoring 20-goals in all competitions, before Chelsea forked out a club-record (£60M, per the Daily Star) fee to secure his services last summer. There are a lot of thoughts in the mind of the coach before preparing the final list, and you have to try to have a very clear mind.
Once the lynchpin of the Chelsea defence, Cahill was left on the bench for both legs of his side's Champions League defeat to Barcelona as well as the games against both Manchester clubs.
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Morata struggled with back injuries earlier this year and last scored for Chelsea on December 26.
He was quoted as saying by L'Equipe: "Admittedly, Alvaro Morata has not scored for a long time, but he is our team-mate and when the team loses, we all lose together".
"We are looking to get more information about players". "But now he is back, although it doesn't mean he has a spot guaranteed (for the World Cup)".
Conte, who used to be the Italian national team manager, has experience of picking players and said these months are vital for any player wanting to go to the World Cup. Gary is a player who for his experience and for the quality of the player and the person, I hope he goes in the national team and plays another World Cup'.
"The fact players haven't been called is down to various things; form, injuries and also to see new players better".